Small glass beaker or cup Procedure Divide the potato into three roughly equal sections. Keep one section raw and at room temperature.
Average final volumes of oxygen for each concentration of hydrogen peroxide. Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total. I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased.
Also, most of the points were on or close to the curve of best fit for each concentration. However, there are some factors that I must take into consideration. Apparatus Limitations Firstly, there were limitations on the apparatus that I used. Each piece of apparatus has an apparatus error with an upper and lower limit.
This obviously affects the amount of catalase present, which means that there could be more or fewer collisions and resulting successful collisions between enzyme and substrate molecules depending on the greater or lower mass of yeast.
For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.
This would result in a greater probability of successful collisions, and therefore more enzyme-substrate complexes being produced. This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0.
The same idea applies to the substrate concentration in that the pipettes also had an apparatus error. This means the amount of substrate could have been different for each repeat, even though I used the same concentration.
So in cm3, the actual volume could have been either If there were fewer molecules of hydrogen peroxide, there would have been fewer collisions between molecules of enzyme and substrate, resulting in fewer enzyme-substrate complexes being made. However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.
Choice of Method I tried to select the method I considered would be most accurate. I decided on the gas syringe method because, as I explained in my section on preliminary work, it measured the volume of gas directly and minimised the volume of oxygen which could potentially dissolve in water.
However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions. Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas.
To prevent this I had to dry out the barrel and syringe before commencing the procedure. It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.
I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test.Catalase and hydrogen peroxide experiment coursework, - Publication editing.
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Aug 12, · In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things.
Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform decomposition, or the breaking down, of other substances/5(). People invited to a presentation do not need a Prezi account; Transcript of Effect of Heat on the Activity of Catalase in Hydrogen Peroxide.
Effect of Temperature on Enzyme Activity (5 were used in this experiment for efficiency)-safety goggles-Yeast% H2O2 solution-Water bath incubator.
Oct 27, · I assume the project is about showing how catalase decomposes hydrogen peroxide under different conditions and then using what you have found to suggest a mechanism for the reaction.
First of all you need to come up with a specification for your project, a monstermanfilm.com: Resolved. An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide.
Introduction This investigation will be carried out to investigate the rate of reaction of the . CATALASE AND HYDROGEN PEROXIDE Introduction My coursework is on catalase activity. This is done using hydrogen peroxide and two independent variables of my choice.
I carried out a preliminary experiment so that I got a basic idea of what I was doing and what to expect.